For me, it was Princess Rosalina’s backstory in Super Mario Galaxy.
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A weird one that I’ve not seen mentioned: the intro to Battlefield 1. It is really well crafted and was emotional in a way I’ve never felt from a war FPS.
The multiple unavoidable deaths and brief epitaph shown on screen, before hot swapping to the next soldier felt poignant. Really hit me with the pointlessness and futility of WWI, the plight of the common soldier and the sheer scale of death.